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  • Design & Pitfalls

    • How good are the algorithms for predicting miRNA binding targets?

    • Alternative approach to generate shRNA from cDNA

    • Retroviral delivery of small interfering RNA into primary cells

    • RNAi cytotoxicity & instability using lentiviral vectors

    • Rational siRNA design for RNAi

    • Alleviating interferon response & maintaining efficacious siRNA response

    • Delivery & Specificity

  • Large Scale/High Content/High-Throughput Screens (HTS)

    • RNAi living-cell microarrays for loss-of-function screens in Drosophila melanogaster cells
    • MicroRNA expression microarray for mammalian brain
    • RNA interference microarrays: high-throughput loss-of-function genetics in mammalian cells
    • siRNA cell arrays for high-content screening microscopy
    • Using microarrays to screen for RNAi
    • Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets
    • Functional Dissection of an Innate Immune Response
    • Genome-wide RNAi analysis of C. elegans fat regulatory genes
    • A large-scale RNAi screen in human cells identifies new components of the p53 pathway
    • Resource for large-scale RNA-interference-based screens in mammals
    • Growth & Viability - Genome-Wide
    • Hedgehog signaling - Genome-scale

  • Post-Transcriptional Pathway/Player updates

    • MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies

    • mRNA Turnover Meets RNA Interference

    • Argonaute and Its Implications for RISC Slicer Activity

    • Argonaute Journeys into the Heart of RISC

    • Argonaute2 Is the Catalytic Engine of RNAi

    • Plant miRNA surprises

    • Overhang-specific small interfering RNA recognition by the PAZ domain

    • RISC: a 5' phosphomonoester-producing RNA endonuclease

    • RNAi takes Evo-Devo World by Storm

    • Human genes hit the big screen

    • Armitage is required for RISC

    • RNase negatively regulates RNAi

    • Sequence-Specific Inhibition of Small RNA Function

  • Transcriptional Pathway/Player updates

    • Methylation mystery: miRNA vs. siRNA - see model

    • RITS acts in cis to promote RNAi-mediated transcriptional and post-transcriptional silencing

    • Induction of DNA methylation and gene silencing by short interfering RNAs in human cells

    • Emergence of alternate pathways

    • SiRNA silencing in human cells

    • Transcriptional Activation by Small dsRNA

    • Repression by non coding RNA

    • Chromatin packaging depends on RNAi

    • Heterochromatin Targeting

    • Transposable elements in heterochromatin and epigenetic control

  • Pathways/Players for transcriptional & posttranscriptional RNAi

    • A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in S. pombe

    • Wnt Signaling Glows with RNAi

  • RNAi Therapeutic Models

    • Reviews

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    • Can companies deliver on the RNAi business?

    • RNAi In Vivo Applications

    • RNAi as a gene therapy approach

    • eiRNAi

    • RNAi turns poppies off morphine

    • A cholesterol connection in RNAi - Therapeutic Silencing of endogenous gene

    • Silencing Cancer - multidrug-resistant

    • Novel RNAi Therapy: Intron-Derived MicroRNA Drugs

    • Tailor-made Riboswitches

• New Publication lists        MM

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  • C. elegans - Fabio Piano's Database

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  • Cell division related genes - Hyman RNAi  

  • miRNA

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    • Drosophila microRNA targets

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  • Phenomic database - model organisms

  • Plant Chromatin DB - RNAi Lines & Vectors

  • Reactome: bio-processes knowledgebase

  • RNA World

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    • Human and Mouse Libraries

    - also see NCI site

    • Alternative approach to generate shRNA from cDNA

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    • Database - Trono lab

    • User guide - Tuschl lab

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  • RNAi Collection - Over 7,200 Drosophila dsDNA constructs

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- also see NCI site

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Vectors developed by AfCS - available at ATCC

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